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CE proteins without the Pathogenesis-Related 1α (PR1α) signal peptide from Nicotiana tabacum were expressed in N. benthamiana leaves using Agrobacterium tumefaciens -mediated transient transformation assays (ATTAs). Total protein was extracted from leaves at 2 d post-agroinfiltration and an anti-FLAG antibody used for the immunodetection of CE proteins. The PageRuler <t>Prestained</t> Protein Ladder (Thermo Fisher Scientific) is shown on the left of each membrane. Asterisks (*) have been placed above CE protein bands. The expected sizes of the CE proteins are 20.4 kDa (CE28), 15.5 kDa (CE33), 21.8 kDa (CE74), 19.2 kDa (CE77), 13.5 kDa (CE89), and 22.4 kDa (CE128).
Precision Plus Proteintm All Blue Prestained Protein Standard, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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CE proteins without the Pathogenesis-Related 1α (PR1α) signal peptide from Nicotiana tabacum were expressed in N. benthamiana leaves using Agrobacterium tumefaciens -mediated transient transformation assays (ATTAs). Total protein was extracted from leaves at 2 d post-agroinfiltration and an anti-FLAG antibody used for the immunodetection of CE proteins. The PageRuler <t>Prestained</t> Protein Ladder (Thermo Fisher Scientific) is shown on the left of each membrane. Asterisks (*) have been placed above CE protein bands. The expected sizes of the CE proteins are 20.4 kDa (CE28), 15.5 kDa (CE33), 21.8 kDa (CE74), 19.2 kDa (CE77), 13.5 kDa (CE89), and 22.4 kDa (CE128).
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CE proteins without the Pathogenesis-Related 1α (PR1α) signal peptide from Nicotiana tabacum were expressed in N. benthamiana leaves using Agrobacterium tumefaciens -mediated transient transformation assays (ATTAs). Total protein was extracted from leaves at 2 d post-agroinfiltration and an anti-FLAG antibody used for the immunodetection of CE proteins. The PageRuler <t>Prestained</t> Protein Ladder (Thermo Fisher Scientific) is shown on the left of each membrane. Asterisks (*) have been placed above CE protein bands. The expected sizes of the CE proteins are 20.4 kDa (CE28), 15.5 kDa (CE33), 21.8 kDa (CE74), 19.2 kDa (CE77), 13.5 kDa (CE89), and 22.4 kDa (CE128).
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CE proteins without the Pathogenesis-Related 1α (PR1α) signal peptide from Nicotiana tabacum were expressed in N. benthamiana leaves using Agrobacterium tumefaciens -mediated transient transformation assays (ATTAs). Total protein was extracted from leaves at 2 d post-agroinfiltration and an anti-FLAG antibody used for the immunodetection of CE proteins. The PageRuler <t>Prestained</t> Protein Ladder (Thermo Fisher Scientific) is shown on the left of each membrane. Asterisks (*) have been placed above CE protein bands. The expected sizes of the CE proteins are 20.4 kDa (CE28), 15.5 kDa (CE33), 21.8 kDa (CE74), 19.2 kDa (CE77), 13.5 kDa (CE89), and 22.4 kDa (CE128).
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CE proteins without the Pathogenesis-Related 1α (PR1α) signal peptide from Nicotiana tabacum were expressed in N. benthamiana leaves using Agrobacterium tumefaciens -mediated transient transformation assays (ATTAs). Total protein was extracted from leaves at 2 d post-agroinfiltration and an anti-FLAG antibody used for the immunodetection of CE proteins. The PageRuler <t>Prestained</t> Protein Ladder (Thermo Fisher Scientific) is shown on the left of each membrane. Asterisks (*) have been placed above CE protein bands. The expected sizes of the CE proteins are 20.4 kDa (CE28), 15.5 kDa (CE33), 21.8 kDa (CE74), 19.2 kDa (CE77), 13.5 kDa (CE89), and 22.4 kDa (CE128).
Precision Plus Protein All Blue Standard, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Bio-Rad precision plus protein all blue prestained protein standards
CE proteins without the Pathogenesis-Related 1α (PR1α) signal peptide from Nicotiana tabacum were expressed in N. benthamiana leaves using Agrobacterium tumefaciens -mediated transient transformation assays (ATTAs). Total protein was extracted from leaves at 2 d post-agroinfiltration and an anti-FLAG antibody used for the immunodetection of CE proteins. The PageRuler <t>Prestained</t> Protein Ladder (Thermo Fisher Scientific) is shown on the left of each membrane. Asterisks (*) have been placed above CE protein bands. The expected sizes of the CE proteins are 20.4 kDa (CE28), 15.5 kDa (CE33), 21.8 kDa (CE74), 19.2 kDa (CE77), 13.5 kDa (CE89), and 22.4 kDa (CE128).
Precision Plus Protein All Blue Prestained Protein Standards, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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precision plus protein all blue prestained protein standards - by Bioz Stars, 2026-03
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Bio-Rad precision plus protein dual color standard
CE proteins without the Pathogenesis-Related 1α (PR1α) signal peptide from Nicotiana tabacum were expressed in N. benthamiana leaves using Agrobacterium tumefaciens -mediated transient transformation assays (ATTAs). Total protein was extracted from leaves at 2 d post-agroinfiltration and an anti-FLAG antibody used for the immunodetection of CE proteins. The PageRuler <t>Prestained</t> Protein Ladder (Thermo Fisher Scientific) is shown on the left of each membrane. Asterisks (*) have been placed above CE protein bands. The expected sizes of the CE proteins are 20.4 kDa (CE28), 15.5 kDa (CE33), 21.8 kDa (CE74), 19.2 kDa (CE77), 13.5 kDa (CE89), and 22.4 kDa (CE128).
Precision Plus Protein Dual Color Standard, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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CE proteins without the Pathogenesis-Related 1α (PR1α) signal peptide from Nicotiana tabacum were expressed in N. benthamiana leaves using Agrobacterium tumefaciens -mediated transient transformation assays (ATTAs). Total protein was extracted from leaves at 2 d post-agroinfiltration and an anti-FLAG antibody used for the immunodetection of CE proteins. The PageRuler Prestained Protein Ladder (Thermo Fisher Scientific) is shown on the left of each membrane. Asterisks (*) have been placed above CE protein bands. The expected sizes of the CE proteins are 20.4 kDa (CE28), 15.5 kDa (CE33), 21.8 kDa (CE74), 19.2 kDa (CE77), 13.5 kDa (CE89), and 22.4 kDa (CE128).

Journal: bioRxiv

Article Title: From Nicotiana to apple: effector screening reveals new avenues for durable scab resistance

doi: 10.64898/2025.12.25.696553

Figure Lengend Snippet: CE proteins without the Pathogenesis-Related 1α (PR1α) signal peptide from Nicotiana tabacum were expressed in N. benthamiana leaves using Agrobacterium tumefaciens -mediated transient transformation assays (ATTAs). Total protein was extracted from leaves at 2 d post-agroinfiltration and an anti-FLAG antibody used for the immunodetection of CE proteins. The PageRuler Prestained Protein Ladder (Thermo Fisher Scientific) is shown on the left of each membrane. Asterisks (*) have been placed above CE protein bands. The expected sizes of the CE proteins are 20.4 kDa (CE28), 15.5 kDa (CE33), 21.8 kDa (CE74), 19.2 kDa (CE77), 13.5 kDa (CE89), and 22.4 kDa (CE128).

Article Snippet: The purified protein samples were resolved alongside the Precision Plus ProteinTM All Blue Prestained Protein Standard (Bio-Rad) for molecular weight estimation.

Techniques: Transformation Assay, Immunodetection, Membrane